Make / Tempeh

Method · Updated on 9 October 2026

How to make tempeh at home

Soak, cook, acidify, dry, inoculate and incubate: a careful home tempeh method, with the acidity and temperature that matter.

You need
Soybeans, tempeh starter, lactic acid
Incubate
24–36 hours at 30 °C (86 °F) 1
Ready when
A firm white cake 5
A small bowl of miso and a sliced block of tempeh

Tempeh is a compact white cake of dehulled, cooked soybeans bound together by Rhizopus mould. 5 It is a controlled mould fermentation, so the starter, the acidity of the beans and the temperature all matter. 6 Tempeh is always cooked before eating. 1

You need

  • Dry soybeans, dehulled (split), or whole beans you dehull after soaking 1
  • Tempeh starter (Rhizopus spores) from a reputable supplier, at the dose on its pack 57
  • Food-grade lactic acid, to bring the beans to pH 4.8 or lower 2

Equipment

  • Large pan and colander
  • pH strips or a pH meter
  • Perforated food bags, or trays with a perforated cover
  • An incubator or warm box that holds 25–30 °C, with a thermometer 1

Method

  1. Soak

    Soak the dehulled beans for 6–24 hours, then discard the soaking water. Do not reuse soak water from an earlier batch. 12

  2. Cook

    Cook the beans in fresh water for 20–30 minutes, then drain and discard the cooking water. 1

  3. Acidify

    Before inoculating, the beans should be at pH 4.8 or lower: add food-grade lactic acid and check with strips or a meter. In tests, lactic acid at this pH controlled Bacillus cereus while vinegar (acetic acid) did not, and unacidified beans fermented poorly. Producers add the acid during soaking or cooking. 21

  4. Dry and cool

    Spread the hot beans out so that surface water steams off; free water encourages spoilage. Cool them to 20–25 °C (68–77 °F). 1

  5. Inoculate

    Mix the starter in evenly at the dose on its pack. Too little gives patchy growth and more spoilage; too much makes the batch run hot. 1

  6. Pack

    Fill perforated bags or containers in a layer 3–5 cm deep, with small, evenly spaced holes: the mould needs some air, but a thicker layer starves the centre. 1

  7. Incubate

    Keep at 30 °C (86 °F) for 24–36 hours, or at 25 °C (77 °F) for 48–72 hours. Once white growth shows, the beans make their own heat and can reach 40–50 °C, too hot for the mould: lower the setting, space the packs apart and let air move. 13

  8. Chill, then cook

    As soon as the cake is ready, refrigerate it at 4 °C (40 °F) or below, or freeze it at −18 °C (0 °F). Always cook tempeh before eating. 41

How to tell it is ready

  • The beans are completely covered in white mycelium and bound into a firm cake that does not fall apart when cut 53
  • It smells fresh and mushroom-like, with no ammonia 5
  • It is creamy white; a few grey or black spots near the air holes are spores and acceptable in small amounts 1

Storing it

  • Fresh tempeh is perishable: refrigerate at 4 °C (40 °F) or below as soon as it is ready 14
  • Freeze it at −18 °C (0 °F) for longer keeping 84
  • We have not found a primary source for how many days home tempeh keeps in the fridge. Cook it soon; if in doubt, throw it out.

And miso?

Miso is a longer project: koji (rice or barley grown with Aspergillus oryzae), cooked soybeans and salt mature together for months. Miso usually holds 11–13% salt, within a range of 5–13%, and matures at 25–30 °C for one month to several years; the salt holds back unwanted microbes and steers the fermentation. More koji makes a sweeter miso. 9 We have not found a primary source for tested home proportions or for handling surface growth on home miso, so we do not publish a home miso method yet. Tempeh takes days rather than months and is the better first project.

Next batch: change one thing

Write down what you did in a Batch Card, change a single variable (time, temperature, starter or ingredient) and compare. Problems? See Tempeh problems and fixes.

Sources

  1. Nout and Kiers (2005), Tempe fermentation, innovation and functionality, Journal of Applied MicrobiologyProcess: dehull, soak 6–24 h, cook 20–30 min, dry, cool, inoculate, pack 3–5 cm in perforated bags, 24–36 h at 30 °C or 48–72 h at 25 °C; self-heating to 40–50 °C; spores with more air; tempe is cooked before eating. Accessed 2026-10-09.
  2. Wickware (2015), Critical parameters for the production of safe soybean tempeh, Cornell University thesisLactic acid to a whole-bean pH of 4.8 or lower controlled Bacillus cereus; acetic acid at the tested pH did not; unacidified beans fermented poorly; backslopping soak water discouraged; sticky shiny beans signalled B. cereus. Accessed 2026-10-09.
  3. Okada (1989), Role of microorganisms in tempeh manufacture, JARQAbout 1–2 days near 27 °C; temperature rises once mould growth is visible; over-ripe tempeh shows spores and ammonia as pH rises above 7.5. Accessed 2026-10-09.
  4. US FDA: Are you storing food safely?Refrigerator at or below 4 °C (40 °F); freezer at −18 °C (0 °F). Accessed 2026-10-09.
  5. Codex Alimentarius: Regional standard for tempe (CXS 313R-2013)Tempe is a compact white cake of dehulled boiled soybeans fermented with Rhizopus; compact when cut, white mycelium, fresh odour without ammonia. Accessed 2026-10-09.
  6. BC Centre for Disease Control: Tempeh guidanceTempeh is a controlled mould fermentation in which Rhizopus grows through the beans; starter, acidity and temperature are the process controls. Accessed 2026-10-09.
  7. Griese et al. (2013), Gastroenteritis outbreak associated with unpasteurized tempeh, Emerging Infectious DiseasesA 2012 outbreak linked to unpasteurised tempeh; the outbreak strain was found in Rhizopus starter culture. Accessed 2026-10-09.
  8. FAO: Edible flours and protein products from soybeans, chapter 9Tempeh ferments for 1–2 days and is highly perishable, preserved by drying or freezing; miso of 8–14% salt matures for weeks to months. Accessed 2026-10-09.
  9. Kusumoto et al. (2021), Japanese traditional miso and koji making, Journal of FungiMiso salt usually 11–13% (range 5–13%); matures at 25–30 °C for one month to years; salt inhibits unwanted microbes; more koji gives a sweeter miso. Accessed 2026-10-09.

Numbers on this page come from these sources. Where sources give a range, we quote the range rather than a single figure.

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